African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12278

Full Length Research Paper

Sampling strategy to develop a primary core collection of apple cultivars based on fruit traits

Zhang Jie1, Wang Yi1, Zhang Xinzhong1, Li Tianzhong1, Wang Kun2, Xu Xuefeng1 and Han Zhenhai*
  1Institute of Horticultural Plants, China Agricultural University, a Key Laboratory of Beijing Municipality of Stress Physiology and Molecular Biology for Fruit, Tree, Beijing 100193, China. 2Fruit Tree Research Institute of the Chinese Academy of Agricultural Sciences, China.
Email: [email protected]

  •  Accepted: 08 May 2009
  •  Published: 11 January 2010



A total of 435 accessions of apple germplasm collected from the Xincheng National Apple Germplasm Repository and 10 morphological traits of them were used for studying the optimal sampling strategy for primary core collection of apple (Malus domestica Brokh). In order to acquire the appropriate primary core collection, different entire sampling ratio and sampling scheme were compared in the study. Six entire sampling ratios were tested and the sampling schemes following stratification into two levels, including the grouping principle, sampling proportion within group were studied. The results showed that 10% should be the suitable entire sampling ratio for primary core collection of apple. Under 15% entire sampling ratio, the optimal sampling scheme was grouped based on cultivar group combining with genetic diversity based sampling proportion within group proportion. This sampling strategy was used to acquire the primary core collection of 64 accessions from 435 accessions of apple cultivars, and the primary core collection could well represent the genetic diversities of the entire variety collection.


Key words: Core collection, sampling strategy, fruit traits, apple.