African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12269

Full Length Research Paper

Antioxidant properties of cultivated edible mushroom (Agaricus bisporus) in Kenya

Grace A. Muna
  • Grace A. Muna
  • Department of Biochemistry and Biotechnology, Kenyatta University, P.O Box 43844, 00200, Nairobi, Kenya.
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Muoma John
  • Muoma John
  • Department of Biochemistry and Biotechnology, Kenyatta University, P.O Box 43844, 00200, Nairobi, Kenya.
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Mwanzia Benson
  • Mwanzia Benson
  • Department of Biochemistry, University of Nairobi, P.O Box 30197, 00100, Nairobi, Kenya.
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Dorington Ogoyi*
  • Dorington Ogoyi*
  • Department of Biochemistry and Biotechnology, Technical University of Kenya, P.O. Box 52428- 00200, Nairobi, Kenya.
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  •  Received: 24 April 2015
  •  Published: 22 April 2015

Abstract

Antioxidant activities and phytochemical compounds of ethanol and hot water extracts of Agaricus bisporus species fruiting body and mycelia cultivated in Kenya were spectophotometrically determined and evaluated. The total antioxidant activity was analysed using 1,1-diphenyl-2-picrylhydrazil, hydroxyl, superoxide radical scavenging and reducing power assays, while phytochemicals were assayed through calorimetric assays. Total phenolic, β-carotene, lycopene, flavanoid and ascorbic acid composition of A. bisporus extracts was analysed by calometric assays and found to contain 40.26 to 4.61 mg/g, 48.99 to 2.86 mg/g, 67.82 to 11.87 mg/g, 93.8 to17.2 mg/g and 11.62 to 10.22 mg/g) respectively. The mineral elemental analysis done using energy dispenser x-ray fluorescence (EDXRF) analytical method revealed that the samples contain zinc (42.9 mg/l), iron (33 to 48.5 mg/l), copper (18 to 24 mg/l) and manganese (7.5 to 9 mg/l). Generally, the mycelium extracts were more effective radical scavengers than the fruiting bodies. Due to the above characteristics, A. bisporus mushroom could be considered a food complement with antioxidative activity in the diet for the health benefits they present. Their effectiveness was also evaluated by their EC50 values through interpolation from linear regression analysis of their respective data.

 

Key words: Button mushroom (Agaricus bisporus), edible mushroom, antioxidant, reducing power, scavenging ability, phytochemicals.

Abbreviation

MEE, Mycelium ethanoic extract; FBEE, fruiting body ethanoic extract; FBHWE, fruiting body hot water extract; MHWE, mycelium hot water extract.