African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

The isolation and identification of predatory bacteria from a Microcystis algal bloom

Jabulani R. Gumbo1,2*, Gina Ross1 and Thomas E. Cloete1
  1Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria 0001, South Africa. 2Department of Hydrology and Water Resources, University of Venda, Thohoyandou, 0950, South Africa.
Email: [email protected], [email protected]

  •  Accepted: 13 August 2009
  •  Published: 01 February 2010

Abstract

 

Water samples were collected from a hypertrophic lake, Hartbeespoort dam, where lytic or predatory bacteria were isolated. The water samples were spread on BG 11 agar plates where upon incubation, plaques were observed on Microcystis lawns. Two isolates were further subjected to screening to evaluate their lytic activities onMicrocystis cells and identification. Isolates B2 and B16 had a lytic effect on theMicrocystis cells with isolate B16 having a greater effect than isolate B2. Isolate B2 was identified as Pseudomonas stutzeri with 99.9% certainty and B16 as Bacillus mycoides with 99.7% certainty using the API system.

 

Key words: Microcystis, predator-prey ratio, Bacillus mycoides, Pseudomonas stutzeri.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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