African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12196

Full Length Research Paper

Molecular characterization of virus isolates from genus Potyvirus infecting Vigna subterranea in Burkina Faso

Konate M. N.
  • Konate M. N.
  • Laboratoire Biosciences, Équipe Génétique et Amélioration des Plantes, Université Ouaga I Pr Joseph KI-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Ouedraogo M.
  • Ouedraogo M.
  • Laboratoire de Génétique et de Biotechnologies Végétales, Département de Productions Végétales, Institut de l’Environnement et de Recherches Agricoles (INERA), 04 BP 8645 Ouagadougou, Burkina Faso.
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Neya B. J.
  • Neya B. J.
  • Laboratoire de Virologie et de Biotechnologies Végétales, Département de Productions Végétales, Institut de l’Environnement et de Recherches Agricoles (INERA), 01 BP 476, Ouagadougou, Burkina Faso.
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Bangratz M.
  • Bangratz M.
  • Institut de Recherche pour le Développement (IRD), 911 Avenue Agropolis, 34394 Montpellier, France.
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Palanga E.
  • Palanga E.
  • Laboratoire Mixte International Patho-Bios, 01 BP 476, Ouagadougou, Burkina Faso.
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Nandkangre H.
  • Nandkangre H.
  • Laboratoire de Génétique et de Biotechnologies Végétales, Département de Productions Végétales, Institut de l’Environnement et de Recherches Agricoles (INERA), 04 BP 8645 Ouagadougou, Burkina Faso.
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Ouoba A.
  • Ouoba A.
  • Laboratoire de Génétique et de Biotechnologies Végétales, Département de Productions Végétales, Institut de l’Environnement et de Recherches Agricoles (INERA), 04 BP 8645 Ouagadougou, Burkina Faso.
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Nanema R.
  • Nanema R.
  • Laboratoire Biosciences, Équipe Génétique et Amélioration des Plantes, Université Ouaga I Pr Joseph KI-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Sawadogo N.
  • Sawadogo N.
  • Laboratoire Biosciences, Équipe Génétique et Amélioration des Plantes, Université Ouaga I Pr Joseph KI-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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Sawadogo M.
  • Sawadogo M.
  • Laboratoire Biosciences, Équipe Génétique et Amélioration des Plantes, Université Ouaga I Pr Joseph KI-Zerbo, 03 BP 7021 Ouagadougou 03, Burkina Faso.
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  •  Received: 26 July 2017
  •  Accepted: 18 September 2017
  •  Published: 27 September 2017

Abstract

Bambara groundnut (Vigna subterranea) is an African legume with a great nutritional, economic and social potential. However, one of the main constraints to this crop is viral diseases which reduced yields. Indeed, approximately 12 viruses have been reported to infect bambara groundnut. Among these, only four were reported from Burkina Faso, namely, cowpea aphid-borne mosaic virus (CABMV), bean common mosaic virus strain-blackeye cowpea mosaic (BCMV-BlCM), peanut mottle virus (PeMoV), and cowpea mottle virus (CPMoV). This study was carried out in order to identify and characterize the main viruses occurring in bambara groundnut from Burkina Faso using serological and molecular tests. 140 plants were sampled in the three agro-climatic zones of Burkina Faso. Both Potyvirus CABMV and BCMV-BlCM were identified in 8.57% of the samples when double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) test was used and 14.29% of the samples when reverse transcription-polymerase chain reaction (RT-PCR) test was used. Phylogenetic tree based on 476 nt in coat protein showed that all Burkina Faso CABMV isolates clustered together with Uganda isolate (KT726938). However, two groups were distinguished within these isolates. Burkina Faso BCMV-BlCM isolates are strengthly clustered with BCMV and BlCMV group. This study reports the first molecular characterization of CABMV and BCMV-BlCM infecting Bambara groundnut in Burkina Faso.

Key words: Bambara groundnut, cowpea aphid-borne mosaic virus (CABMV), bean common mosaic virus strain-blackeye cowpea mosaic (BCMV-BlCM), double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA), reverse transcription-polymerase chain reaction (RT-PCR).