African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Isolation of filamentous fungi present in swine wastewater that are resistant and with the ability to remove atrazine

Morgana S. Gonçalves1*, Silvio C. Sampaio2, Luciane Sene2, Floriano L. Suszek2, Silvia R. M. Coelho2 and Claudia E. C. Bravo1
1Coordination of Environmental Engineering Course, Federal University of Technology - Paraná, Francisco Beltrão, Paraná, Brazil. 2Postgraduate Program in Agricultural Engineering, Western Paraná State University, Cascavel, Paraná, Brazil.  
Email: [email protected]

  •  Accepted: 06 June 2012
  •  Published: 21 June 2012

Abstract

In this work, we isolated and identified filamentous fungi present in swine wastewater (SW) that are resistant and with the ability to remove atrazine removal. For the isolation, the SW was inoculated into liquid medium containing 0.01 and 0.1 g L-1 of atrazine and after the adaptation period, was transferred on solid medium containing 10 mg L-1 atrazine. Three strains of filamentous fungi were isolated and identified asCladosporium cladosporioideRhizopus stolonifer and Penicillium purpurogenum. The isolates were tested for their ability to degrade atrazine in liquid medium containing 1.5 g L-1 of the herbicide as single source of carbon and nitrogen. In 15 days of culture, the isolated R. stolonifer showed a higher removal rate (73.75%), followed by P. purpurogenum (73.42%) and C. cladosporioides (68.42%), indicating a high possibility of using these microorganisms in the removal of atrazine in waters and effluents.

 

Key words: Atrazine, swine, filamentous fungi.

Abbreviation

SW, Swine wastewater.