African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Isolation and characterization of rock phosphate solubilizing actinobacteria from a Togolese phosphate mine

  Hanane Hamdali1, 2, Koriko Moursalou3, Gado Tchangbedji3, Yedir Ouhdouch4* and  Mohamed Hafidi1  
  1Faculté des Sciences Semlalia, Université Cadi Ayyad (UCAM), Laboratoire d’Ecologie & Environnement, Marrakech Morocco. 2Faculté des Sciences et Techniques, Université Sultan Moulay Slimane, Laboratoire de Valorisation et Sécurité des Produits Agroalimentaires, Béni Mellal Morocco. 3Faculté des Sciences Université de Lomé, Laboratoire de Gestion, Traitement et Valorisation des Déchets BP 1515 Lomé Togo. 4Faculté des Sciences Semlalia, Université Cadi Ayyad (UCAM), Laboratoire de Biologie et de Biotechnologie des Microorganismes, Marrakech 40000 Morocco.
Email: [email protected]

  •  Accepted: 16 December 2011
  •  Published: 05 January 2012

Abstract

 

A phosphate mine from Togo, an original biotope rich in insoluble rock phosphate (RP), was explored for the presence of RP solubilizing actinobacteria. One hundred and fifty actinobacteria isolates originating from this mine was tested for their ability to grow on a synthetic minimum medium (SMM) containing insoluble RP as unique phosphate source. Only 29 isolates (19%) were able to weather RP in SMM medium. Five isolates showed the most active growth and solubilization capability. These isolates were shown to be able to solubilize RP in liquid cultures. The study of mechanisms involved in these weathering processes indicated that the isolates produce siderophores but not organic acids. Four of these strains were shown to belong to the genus Micromonospora and one, to the genus Streptomyces.

 

Key words: Actinobacteria, isolation, characterization, Togolese phosphate mine, rock phosphate solubilization.

Abbreviation

RP, Rock phosphate; SMM, synthetic minimum medium.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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