African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Optimization of Newcastle disease virus production in T-flask

Mohd Azmir Arifin1, Maizirwan Mel1*, Siti Hajar Salim1, Mohamed Ismail Abdul Karim1 and Sharifah Syed Hassan2
1Department of Biotechnology Engineering, Kulliyyah of Engineering, International Islamic University Malaysia, Gombak, P.O. Box 10, 50728 Kuala Lumpur, Malaysia. 2School of Medicine and Health Sciences, Monash University, Sunway Campus, Jalan Lagoon Selatan, Bandar Sunway, 46150 Selangor, Malaysia
Email: [email protected]

  •  Accepted: 07 November 2011
  •  Published: 16 December 2011

Abstract

Newcastle disease (ND) is regarded as one of the most important diseases in the poultry industry. Currently, vaccines for ND are produced by using embryonated chicken eggs, a method which has the disadvantages of being labour-intensive, time consuming and requires large area for the incubation of eggs. In the present study, the production of lentogenic Asplin F strain of Newcastle disease virus by using cell culture method was studied. Experiments were carried out in T-flasks to investigate the effects of serum concentration in the culture medium during virus replication phase and multiplicity of infection (MOI) on ND virus propagation in DF-1 cells. Results show that virus infectivity titre of 6.62 x 107 TCID50/ml was achieved when serum concentration of 0.5% and MOI of 20 was used.

 

Key words: Newcastle disease virus, Asplin F strain, DF-1 cell, T-flask

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This article is published under the terms of the Creative Commons Attribution License 4.0

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