Abstract

Molecular characterization of thirteen common bean genotypes was done with random amplified polymorphic DNA (RAPD) markers. Initially, 15 primers were screened out of which only seven were selected which generated a total of 65 amplification products out of which 63 bands (96.62%) were polymorphic indicating fair amount of polymorphism. The genotypes shared 43% genetic similarity among themselves. Cluster analysis delineated the genotypes into three groups with seven, five and one genotype in cluster-I, II and III, respectively. The maximum similarity index (82.35) based dice similarity coefficient was obtained between SKUA-R-21 and SKUA-R-19, while it was minimum (27.72) between genotypes PBG-29 and SKUA-R-01.

Key words: Genetic divergence, common bean, random amplified polymorphic DNA (RaPD).

Abbreviation

Abbreviations: RFLP, Restriction fragment length polymorphism; RAPD, random amplified polymorphic DNA; CTAB, cetyl trimethyl ammonium bromide; PCR, polymerase chain reaction.