African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Molecular variance of the Tunisian almond germplasm assessed by simple sequence repeat (SSR) markers

Hassouna Gouta1*, Elhem Ksia2, Ahmed Mliki3 and Yolanda Gogorcena4
1Department of Genetic Resources and Breeding. Olive Tree Institute. B. P. 1087-3000 Sfax, Tunisia. 2Laboratory of Biology and plants Biotechnology. Faculty of Sciences, Campus Universitary, El Manar 1060 Tunis, Tunisia. 3Laboratory of Molecular Biology of Vine. Center of Biotechnology Borj-Cedria, B.P. 901 Hammam-Lif, 2050 Tunisia. 4Departament of Pomology, Experimental Station of Aula Dei (CSIC), P.O. Box 13034, E-50080 Zaragoza, Spain.  
Email: [email protected]

  •  Accepted: 12 July 2013
  •  Published: 31 July 2013

Abstract

The genetic variance analysis of 82 almond (Prunus dulcis Mill.) genotypes was performed using ten genomic simple sequence repeats (SSRs). A total of 50 genotypes from Tunisia including local landraces identified while prospecting the different sites of Bizerte and Sidi Bouzid (Northern and central parts) which are the most important locations of almond diversity in Tunisia were included. Analysis of molecular variance (AMOVA) was performed for 11 populations from these different regions and foreign countries to examine the distribution of genetic variation of the accessions studied. Results show that the major variation occurred within populations in each geographic site. Additionally, this analysis demonstrates that the genetic diversity within local almond cultivars was important, with a clear geographic distinction between the Northern and the Southern Tunisian cultivars. The value of prospecting new sites, preserving genetic diversity and encouraging on farmers almond collections is also discussed.

Key words: Prunus dulcis Mill., Genetic resources, AMOVA, local ecotypes, geographic origin, Tunisia.

Abbreviation

Abbreviations: UPGMA, Unweighted pair group method with arithmetic mean;AMOVA, analysis of molecular variance; SSR, simple sequence repeat.