African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Genetic variation within and among five natural populations of endangered Sclerocarya birrea (A. Rich) subsp. Birrea in Sudan

Rehab M. Abdelkheir1, Nada B. Hamza2*, Ayoub M. Khalil1 and Essam I. Warrag3        
1Department of Forestry, College of Natural Resources and Environmental Studies, University of Juba, P. O. Box: 321/1, Khartoum, Sudan. 2Department of Molecular Biology, Commission for Biotechnology and Genetic Engineering, National Centre for Research, P. O. Box: 2404, Khartoum, Sudan. 3Faculty of Forestry, University of Khartoum, Post Code 11115, P. O. Box: 321 Khartoum, Sudan.
Email: [email protected]

  •  Accepted: 06 May 2011
  •  Published: 20 June 2011

Abstract

Knowledge of genetic diversity is important for successful conservation and domestication of species. In order to determine genetic diversity within and amongSclerocarya birrea (A. Rich.) Htochst. subsp. birrea populations in Sudan, random amplified polymorphic DNA (RAPD) markers were used. Leaf materials from 75 seedlings from five populations (Rashad, Alfaid, Alkhwi, Aldamazin and Baw) of this species were used to compare the genetic diversity. A total of 37 bands were generated using four primers. Genetic variation within the populations as estimated by Shannon information index ranged from 0.343 to 0.272 with an overall diversity of 0.306. Analysis of molecular variance revealed that 46% of the variation was attributed to differences among the populations and 54% within the populations (P < 0.001). The gene flow among population was small (Nm = 0.297). UPGMA cluster and principal coordinate analyses (PCA) indicated Alfaid as the most distinct population. Since genetic variation was found to occur among and within the populations of S. birrea (A. Rich.) Hochst. Subsp. birrea, conservation of these populations would help in the maintenance of the species.

 

Key words: Sclerocarya birrea subsp. birrea, genetic variation, conservation, random amplified polymorphic DNA (RAPD), Sudan.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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