African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Characterization of wheat varieties by seed storage-protein electrophoresis

Mohd Shuaib1, Alam Zeb1,2*, Zahir Ali1, Waqar Ali1, Taufiq Ahmad3 and Ikhtiar Khan2
1Department of Biotechnology, University of Malakand, Chakdara, Pakistan. 2Institute of Chemical Sciences, University of Peshawar, Peshawar, Pakistan. 3Nuclear Institute for Food and Agriculture (NIFA) Tarnab Peshawar, Pakistan.
Email: [email protected]

  •  Accepted: 05 February 2007
  •  Published: 05 March 2007

Abstract

Wheat grains of thirteen varieties were collected from different ecological regions of Pakistan. The variability of seed storage-proteins was analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Electrophorogram for each variety were scored and Jaccard‘s similarity index (JSI) was calculated. Genetic diversity of wheat was evaluated by constructing the dendrogram for high molecular weight (HMW) and low molecular weight (LMW) gluten subunit bands. It is concluded that seed storage protein profiles could be useful markers in the studies of genetic diversity and classification of adapted cultivars, thereby improving the efficiency of wheat breeding programs in cultivar development especially in a developing country like Pakistan.

 

Key words: Wheat varieties, SDS-PAGE, genetic diversity and cluster analysis

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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