African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Effects of cold-stratification, gibberellic acid and potassium nitrate on seed germination of yellow gentian (Gentiana lutea L.)

Fadil Millaku1, Bekim Gashi1*, Kasamedin Abdullai1, Sali Aliu2, Mirsade Osmani2, Elez Krasniqi1, Valbona Mata3 and Agim Rysha4  
1Department of Biology, Faculty of Mathematical and Natural Sciences, University of Pristina, Republic of Kosovo. 2Deparment of Crop Science, Faculty of Agriculture, University of Pristina, Republic of Kosovo. 3Department of Biotechnology, Faculty of Natural Sciences, University of Tirana, Albania. 4Department of Organic Food Quality and Food Culture, Faculty of Organic Agricultural Sciences, University of Kassel, Germany.  
Email: [email protected]

  •  Accepted: 08 August 2012
  •  Published: 23 August 2012

Abstract

The purpose of this study was to show the effect of cold-stratification, gibberellic acid and potassium nitrate on seed germination of yellow gentian (Gentiana lutea L.). The seeds of natural yellow gentian populations of the Albanian Alps (Kosovo) were collected in September 2010. Seeds placed in filter paper (plastic bag) and in mixture of sand-soil were cold-stratified (2±1°C) for 72 days. The effect of different concentrations of gibberellic acid (250, 500 and 1000 ppm GA3) and potassium nitrate (0.1, 0.2 and 0.3% KNO3) in final germination percentage (FGP), mean germination time (MGT) and germination index (GI) in non-stratified and stratified seeds were examined. Seeds stratified in filter paper and treated with 1000 ppm GA3 and 0.1% KNO3 showed significantly higher percentage of germination (FGP and GI), while seeds stratified in mixture of sand-soil showed the highest values of FGP and GI in treatments with 500 ppm GA3 and different concentrations of KNO3. The cold-stratification and GA3 treatments of yellow gentian seeds are suitable for the higher percentage of germinations.

 

Key words: Gentiana lutea L., cold-stratification, seeds, germination, gibberellic acid (GA3), potassium nitrate (KNO3).

Abbreviation

Abbreviations: FGP, Final germination percentage; GI, germination index; MGT,mean germination time; GA3, gibberellic acid; KNO3, potassium nitrate.