African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Generation and analysis of cDNA library from lipopolysaccharide-stimulated gastropod abalone (Haliotis diversicolor supertexta) hemocytes

Liuji Wu1,3, Li Wang2 and Xinzhong Wu3*
1College of Agronomy, Henan Agricultural University, 63 Nongye Road, Zhengzhou 450002, P. R. China. 2Key Laboratory of Animal Genetics and Breeding of State Ethnic Affairs Commission and Ministry of Education, College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China. 3Laboratory of Marine Life Science and Technology, College of Animal Sciences, Zhejiang University, 268 Kaixuan Road, Hangzhou 310029, P. R. China.
Email: [email protected]

  •  Accepted: 13 June 2011
  •  Published: 03 August 2011

Abstract

A cDNA Library of lipopolysaccharide-stimulated gastropod abalone hemocytes was constructed using switching mechanism at 5' end of RNA transcript (SMART) approach. 300 random clones were selected and sequenced. 180 ESTs was gained. After assembling, we obtained 24 contigs and 119 singlets finally, some of which were immune- related genes. These immune-related genes include cytidine deaminase, ferritin, nonmuscle myosin essential light chain, cytochrome c oxidase subunit I, CD63 antigen-like protein and lysosomal-associated transmembrane protein. This study may contribute to the understanding of the immune mechanism of gastropod abalone Haliotis diversicolor supertexta.

 

Key words: Haliotis diversicolor supertexta, cDNA Library, gene expression,immune mechanism.

Abbreviation

PRPs, Pattern recognition proteins; LPS, lipopolysaccharide.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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