African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Genetic diversity among four Eucalyptus species (myrtaceae) based on random amplified polymorphic DNA (RAPD) analysis

  Osman, A. K.1*, Abd El-Mageed, A. A.2, Tawfik, A. Q.1 and Mohammed, H. A.2          
  1Botany Department, Faculty of Science (Qena), South Valley University, Egypt. 2Flora and Phyto-Taxonomy Research Department, Horticulture Research Institute, Agricultural Research Center, Egypt.
Email: [email protected], [email protected]

  •  Accepted: 27 February 2012
  •  Published: 13 March 2012

Abstract

 

Results obtained from random amplified polymorphic DNA (RAPD) analysis of four species of the genus Eucalyptus revealed that the number of fragments amplified for each of the five studied primer varied between 13 and 14 fragments and their size ranged from 95 to 1055 bp. The total number of bands was 67, total number of monomorphic bands was 32, total number of polymorphic bands was 35, and the polymorphism percentage was 52.2%. The results indicate that 16 positive and 13 negative markers were detected. The marker fragments size ranged between 175 to 630 bp for the negative markers and 235 to 945 bp for the positive markers.

 

Key words: Eucalyptus, random amplified polymorphic DNA (RAPD), genetic diversity, DNA fingerprinting PCR,  dendrogram.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 11 No. 21
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES