African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12240

Full Length Research Paper

Characterization of biochemical behavior of sorghum (Sorghum bicolor [Moench.]) under saline stress conditions using multivariate analysis

Luma Castro de Souza
  • Luma Castro de Souza
  • Departamento De Solos E Adubos, Universidade Estadual Paulista Júlio de Mesquita Filho/UNESP, Brazil
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Mara Regina Moitinho
  • Mara Regina Moitinho
  • Departamento De Solos E Adubos, Universidade Estadual Paulista Júlio de Mesquita Filho/UNESP, Brazil
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Risely Ferraz de Almeida
  • Risely Ferraz de Almeida
  • Departamento De Solos E Adubos, Universidade Estadual Paulista Júlio de Mesquita Filho/UNESP, Brazil
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Ellen Gleyce da Silva Lima
  • Ellen Gleyce da Silva Lima
  • Departamento De Produção Vegetal, Universidade Federal Rural da Amazônia, Brazil
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Leane Castro de Souza
  • Leane Castro de Souza
  • Departamento De Produção Vegetal, Universidade Federal Rural da Amazônia, Brazil
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Myriam Galvão Neves
  • Myriam Galvão Neves
  • Departamento De Produção Vegetal, Universidade Federal de Santa Maria, Santa Maria, Brazil
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Cândido Ferreira de Oliveira Neto
  • Cândido Ferreira de Oliveira Neto
  • Universidade Federal Rural da Amazônia, departamento de Produção Vegetal, Brazil
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Glauco André dos Santos Nogueira
  • Glauco André dos Santos Nogueira
  • Universidade Federal Rural da Amazônia, departamento de Produção Vegetal, Brazil
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Maria Eunice Lima Rocha
  • Maria Eunice Lima Rocha
  • Departamento De Produção Vegetal, Universidade Federal Rural da Amazônia, Brazil
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Mayra Taniely Ribeiro Abade
  • Mayra Taniely Ribeiro Abade
  • Departamento De Produção Vegetal, Universidade Federal Rural da Amazônia, Brazil
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Marlison Tavares Ávila
  • Marlison Tavares Ávila
  • Departamento De Produção Vegetal, Universidade Federal Rural da Amazônia, Brazil
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  •  Received: 04 October 2015
  •  Accepted: 18 April 2016
  •  Published: 04 May 2016

Abstract

The aim of this research was to characterize the biochemical behavior of sorghum plants under saline stress using multivariate statistical analysis methods for efficient management of Sorghum bicolor [Moench.]). The experimental design was completely randomized design composed of three saline concentrations (0, 1.5 and 2.0 M) in 10 replications. In the multivariate analysis (hierarchical method), there were distinct and sub-groups in the sorghum plant treatments. Group 1 consisted of the root parts and under this group there were two subgroups: 1.5 to 2.0 concentration (Group 1) and 2 concentration (Group 2). The increase of NaCl concentration in the roots and leaves has inverse correlation with decrease of nitrate reductase, amino acids, protein and starch. The amounts of amino acids, carbohydrates, sucrose and proline in the roots and carbohydrates, sucrose and proline in the leaves of sorghum plants are reliable biological indicators of saline stress conditions in the soil. The nitrate compound differed (p ≤ 0.05) in the sorghum plant roots; it had an average value of 0.04 μmol kg-1 of nitrate in the control treatment dry matter. The nitrate average was between 0.04 and 0.06 μmol kg-1, but without statistical difference for all concentrations.

 

Key words: Multivariate statistics, salt concentration, proline, carbohydrate.