African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12294

Full Length Research Paper

Somatic embryogenesis in two Iranian date palm cultivars

Parisa Eshraghi1,2, Reza Zarghami3*, Hamideh Ofoghi4 and Mitra Mirabdulbaghi5
1Department of Agriculture, Iranian Research Organization for Science and Technology, Iran. 2Department of Biology, Khatam University, Iran. 3Agricultural Biotechnology Researching Institute of Iran, Mahdasht Road, Karaj, P.O.Box: 31535-1897, Iran. 4Department of Biotechnology, Iranian ResearchOrganization for Science and  Technology, Iran. 5Department of Horticulture, Seed and Plant Improvement Institute, Iran.
Email: [email protected]

  •  Accepted: 02 August 2005
  •  Published: 30 November 2005

Abstract

Shoot tips were removed from 3 to 4 year-old offshoots of adult date palm (Phoenix dactylifera L. cv. Khanizi and Mordarsing) and were cultured on medium that consisted of Murashige and Skoog basal salts medium. After 12 months they were transferred to three different treatments of growth regulators. Five months later, cv. Khanizi produced embryogenic callus on a medium containing 453 µM 2,4-dichlorophenoxyacetic acid (2,4-D), 15 µM N2-(2-isopentenyl)adenine (2ip), and 13 µM 6-benzyl amino purine. This callus was subcultured to another medium that was supplemented with 54 µM NAA and 148 µM 2ip. At this stage the embryos grew into plantlets. The cv. Mordarsing explants, on a medium containing 679 µM 2,4-D and 15 µM 2ip, produced embryogenic callus but the embryos remained in the globular stage.

 

Key words: Callus, in vitro tissue culture, offshoot, Phoenix dactylifera, regenerant.

Abbreviation

Abbreviations: BAP, 6-Benzyl amino purine; cv., cultivar; 2,4-D, 2,4-dichlorophenoxyacetic acid; MS, Murashige and Skoog (1962) media; 2ip, N2-(2-isopentenyl) adenine.