Abstract

RNA extraction is the foundation of modern molecular biology. It is difficult to extract high quality RNA from grapevine tissues, especially in cases where polysaccharides and polyphenols are rich. In this paper, the modified cetyltrie thylammnonium bromide (CTAB), sodium dodecyl sulphate (SDS) and guanidine isothiocyanate methods were applied to extract high purity and integrity RNA from grapevine green and mature berry skins. Highly-grade RNA was isolated by using modified CTAB method and it is suitable for the demands of further molecular biological research such as reverse-transcription polymerase chain reaction (PCR) (RT-PCR), cDNA library construction and Northern hybridization.

Key words: Grapevine berry skins, RNA extraction, reverse-transcription polymerase chain reaction (RT-PCR).

Abbreviation

DEPC, Diethyl pyrocarbonate; EDTA, ethylene diamine tetra-acetic acid; RT-PCR, reverse transcription-polymerase chain reaction; SDS, sodium dodecyl sulphate; CTAB, cetyltrie thylammnonium bromide.