Full Length Research Paper
Abstract
The existence of a target DNA fragments associated with the potential high level ofcatechins in gambier plant was carried out preliminary using RAPD markers. Targetfragment was ligated and transformed into Escherichia coli DH5α strains usingpGem-T Easy vector. The purpose of this study was to obtain information of specificDNA fragments and to generate specific primer combinations associated with potentially high content of catechins. DNA sequence was successfully generated with206 nucleotides in length. The combination of specific primers, designated as Udtg3(5'-CAGATCCTTTGCCACACTGA-3') and Udtg4 (5'- CGTACCTGCCAA CATA ACAG- 3') were successfully designed and could be applied as a diagnostic marker indetection of catechin content production of gambier plant.
Key words: Gambier, RAPD, specific primers, MAS, catechin.
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