African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Isolation and partial purification of lysozyme from saliva of Bali cattle (Bos sondaicus) using an aqueous mixture of polyethylene glycol (PEG) with sodium sulfate

  Sulaiman Ngongu Depamede1,3*, Niken Asri2, Nur Indah Julisaniah2, Bambang F. Suryadi2, and Djoko Kisworo1  
  1Microbiology and Biotechnology Laboratory, Faculty of Animal Sciences, University of Mataram, Jalan Majapahit No. 62, Mataram 83125, Indonesia. 2Faculty of Mathematics and Life Sciences, Mataram University, Jl. Majapahit No. 62 Mataram 83125, Indonesia. 3Immunobiology Laboratory, Faculty of Medicine, University of Mataram, Mataram, Indonesia.
Email: [email protected]

  •  Accepted: 31 October 2011
  •  Published: 26 January 2012

Abstract

 

Lysozyme from saliva of Bali cattle (Bos sondaicus) was successfully isolated through an aqueous mixture system of polyethylene glycol (PEG 4000) and salt (Na2SO4). About 100 µg/ml lysozyme of 14.2 kDa, determined through sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), per 4800 µg/ml saliva was obtained. Based on turbidity assay of Staphylococcus aureus, it was revealed that inhibition activities of 40 µg/ml isolated lysozyme were comparable to 800 µg/ml of a commercial hen egg white lysozyme.

 

Key words: Lysozyme, Bali cattle, Bos sondaicus, PEG, aqueous mixture system.

Abbreviation

 

PEG, polyethylene glycol; ddW, double distillated water; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis.

 

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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