Full Length Research Paper
Abstract
Sulfadimethoxine (SDM) is an antibiotic commonly added to animal feeds. Anti-SDM antibodies are useful for the detection of residual SDM in foods, feeds and biological fluids by ELISA. In this study, we show that SDM is immunogenic in rabbits when it is conjugated with soy 11S globulin or with β-amylase. Rabbit anti-SDM antibodies obtained by immunization with SDM-11S and SDM-beta-amylase displayed low cross-reactivity against other sulfonamides, including sulfamethazine (SM2), sulfasulfonamides (SN), sulfadiazine (SD), sulfamethoxypyrazine (SMP), sulfalene (SMZ), sulfaquinoxaline (SQX). Thus, soy 11S globulin and β-amylase are suitable carriers for the induction of anti-SDM antibodies and have the advantage of being cheaper that BSA.
Key words: Sulfadimethoxine (SDM), sulfasulfonamides (SN), sulfadiazine (SD), sulfamethazine (SM2), sulfamethoxypyrazine (SMP), sulfalene (SMZ), sulfaquinoxaline (SQX), rabbit anti-SDM antibodies, soy 11S globulin, SDM-beta-amylase.
Abbreviation
SDM, Sulfadimethoxine; SN, sulfasulfonamides; SD, sulfadiazine; SM2, sulfamethazine; SMP, sulfamethoxypyrazine; SMZ, sulfalene; SQX,sulfaquinoxaline; DNP, 2,4-dinitrophenyl; BPO, benzylpenicilloyi; BaA, bacterial α-amylase; TAA,taka-amylase A; BSA, bovine serum albumin; HSA, human serum albumin; KLH, keyhole limpet hemocyanin
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