African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

In vitro conservation of Ceropegia elegans, an endemic plant of South India

P. V. Krishnareddy and T. Pullaiah*
Department of Botany, Sri Krishnadevaraya University, Anantapur‐515 003, Andhra Pradesh, India.
Email: [email protected]

  •  Accepted: 22 March 2012
  •  Published: 31 July 2012

Abstract

The purpose of this study was to develop in vitro techniques for conserving endemic species, Ceropegia elegans by axillary shoot multiplication. Murashige and Skoog (MS) medium with kinetin (Kn) 23.20 μM + indole-3-acetic acid (IAA) 5.71 μM was the best for axillary bud proliferation inducing a mean of 7.11 ± 0.07 shoots per node. Shoots developed were rooted best on half strength MS medium with 4.90 μM indole-3-butyric acid (IBA). Plantlets established in pots exhibited 72% survival rate.

 

Key words: Ceropegia elegans, endemic species, micropropagation.

Abbreviation

IAA, Indole-3-acetic acid; IBA, indole-3-butyric acid; Kn, kinetin; MS,Murashige and Skoog (1962) medium; NAA, naphthalene acetic acid; TDZ,thidiazuron; 2-iP, 2-isopentyladenine; 2,4-D, 2,4-dichlorophenoxy acetic acid.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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