African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Evaluation of nutritional components by Plackett-Burman design for Penicillium citrinum lipase production using palm oil mill effluent

Aliyu Salihu1,2, Md. Zahangir Alam1*, M. Ismail AbdulKarim1 and Hamzah, M. Salleh1
1Bioenvironmental Engineering Research Unit (BERU), Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia (IIUM), 50728 Kuala Lumpur, Gombak, Malaysia. 2Department of Biochemistry, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
Email: [email protected] or [email protected]

  •  Accepted: 07 November 2011
  •  Published: 16 December 2011

Abstract

A number of medium components influencing lipase production by Penicillium citrinum (ATCC 42799) were studied using palm oil mill effluent (POME) as the basal medium. The medium components (peptone, yeast extract, malt extract, NH4Cl, NaNO3, KH2PO4, CaCl2, MgSO4, olive oil and Tween-80) were analyzed in twelve experimental trials using Plackett–Burman (PB) design. The most significant components affecting lipase production were found to be Tween-80, peptone, yeast extract, malt extract and NaNO3 at p < 0.05. The results indicate the efficiency of using PB design for screening processes. However, optimal concentration of the significant components can be determined by further statistical analysis.

 

Key words: Lipase, Penicillium citrinum, Plackett–Burman design, palm oil mill effluent

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This article is published under the terms of the Creative Commons Attribution License 4.0

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