African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Genetic variation and relationships of Zea mays and Sorghum species using RAPD-PCR and SDS-PAGE of seed proteins

Osman, G. 1,2* Munshi, A.1 Altf, F. 3 and Mutawie, H.1
1Biology Department, Faculty of Science, Umm-Al-Qura University, Makka 673, Saudi Arabia. 2Agricultural Genetic Engineering Research Institute (AGERI) - (ARC), Giza, Egypt. 3Faculty Of Medicine, Umm-Al-Qura University, Makka 673, Saudi Arabia.  
Email: [email protected]

  •  Accepted: 28 June 2013
  •  Published: 31 July 2013

Abstract

Genetic relationship between some species of Zea mays and Sorghum was determined using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of seed protein and random amplification of polymorphic DNA (RAPD-PCR) markers. According to SDS-PAGE analysis, 78 band were identified across the studied species. The number of bands varies from 17 bands in sample number 5 to 6 in sample number 6. Analysis of RAPD-PCR of DNA provided more precise information concerning relationships between Zea mays and Sorghum species than SDS-PAGE analysis. A remarkable result from this study was identifying a close relationship between Zea mays spp mays and Zea mays spp Mexicana. Further support comes from the molecular data of RAPD, which indicate that close relationship between Sorghum valgare and Sorghum bicolor.

Key words: Zea mays, Sorgum volgare pres, Punciu milia ceaum L. protein, random amplification of polymorphic DNA.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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