Antibiogram of clinical isolates from four wards in a government hospital in Port Harcourt, Nigeria was investigated. Swab and air samples were obtained from patients, personnel, formites and air in orthopaedic, medical, surgical and paediatric wards. A total of 56 isolates were obtained of which Staphylococcus epidermidis (22) was the highest. This was followed by Staphylococcus aureus (16), Streptococcus spp. (5),Escherichia coli (4) and Klebsiella pneumonia (3). Proteus spp., Enterobacter aerogenes and Bacillus cereus had 2 strains each isolated. The Gram positive bacteria were more resistant to norfloxacin, floxapen, and ciprofloxacin but very sensitive to gentamycin, lincocin, rifampicin and streptomycin. S. aureus accounted for the highest resistance to ampiclox followed by S. epidermidis to ciprofloxacin and norfloxacin. The Gram negative bacilli showed highest resistance to ampicillin followed by augumentin, ceporex, and nalidixic acid whereas they were more sensitive to tarivid, peflacin and streptomycin. It could be inferred from the results that patients in this hospital might be at the risk of being infected with antibiotic resistant strains during admission.

Key words: Antibiogram, nosocomial, Port Harcourt, clinical isolates, gram positive bacteria, gram negative bacteria.