African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Development of specific simple sequence repeat (SSR) markers for non-pollen type thermo-sensitive genic male sterile gene in rice (Oryza sativa L.)

Weerachai Matthayatthaworn, Prapa Sripichitt, Chalermphol Phumichai, Sarawut Rungmekarat, Saovaluck Uckarach and Tanee Sreewongchai*
Department of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand
Email: [email protected]

  •  Accepted: 24 October 2011
  •  Published: 21 November 2011

Abstract

A key factor of two-line hybrid rice production is the development of thermo-sensitive genic male sterility (TGMS) lines. In this study, a TGMS line showing non-pollen type thermo-sensitive genic male sterility was used. Crossing between TGMS line (female parent) and normal pollen varieties; CNT1 and PTT1 (male parent) were performed and F1 and F2 populations were develop for each crosses. The phenotypic segregation ratio, 3:1 (fertile: sterile pollens) observed in F2 populations of TGMS/CNT1 and TGMS/PTT1 crosses confirmed that the non-pollen type thermo-sensitive genic male sterility is controlled by a single recessive gene. The bulk segregant analysis (BSA) using simple sequence repeat (SSR) markers were deployed to identify the location and genetic effect of this gene. We have generated new set of SSR markers to identify progenies carrying TGMS gene in a cross TGMS/PTT1. The TGMS gene was located on chromosome 2 with 0.0 cM distance from T2 marker. This TGMS gene was located in the same region of previous identified tmsX gene. It might be the same allele of this gene of 2 different lines. This marker could be used for marker assisted backcrossing to transfer TGMS gene for developing female parent in two line hybrid rice system.

 

Key words: Hybrid rice, thermo-sensitive genic male-sterile, non-pollen type, molecular marker, marker assisted backcrossing.

Abbreviation

BSA, Bulk segregant analysis; CMS, cytoplasmic male sterility;CSPT, critical sterility point temperature; PAGE, polyacrylamide gel electrophoresis;PCR, polymerase chain reaction; SSR, simple sequence repeat; TGMS, thermo-sensitive genic male sterility.