African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12400

Full Length Research Paper

Utilisation of organic and inorganic nitrogen sources by Scleroderma sinnamariense Mont. isolated from Gnetum africanum Welw.

Eneke Esoeyang Tambe Bechem
Department of Botany and Plant Physiology, Faculty of Science, University of Buea, P. O. Box 63 Buea, Cameroon
Email: [email protected]

  •  Accepted: 30 April 2012
  •  Published: 10 May 2012

Abstract

 

The ability of Scleroderma sinnamariense Mont. to utilise some organic and inorganic nitrogen (N) 
sources for growth was examined in axenic liquid cultures. Pisolithus tinctorius Pers. was included for 
comparison. Both fungi species produced measurable biomass on all the N sources used in this 
experiment. The growth of S. sinnamariense on ammonium-N was better than that observed on glycine 
and bovine serum albumin (BSA), comparable to that on glutamic acid and nitrate, but inferior to that 
observed on arginine, alanine and peptone. P. tinctorius showed optimal growth on ammonium-N, 
which was comparable to alanine and glutamic acid but greater than that observed on glycine, arginine, 
BSA, nitrate and peptone. This Scleroderma isolate thus has the potential to utilise nitrogen from 
organic substrates and this may be important for the nitrogen nutrition of its ectomycorrhizal host 
plants in tropical forests. 
 
Key words: Scleroderma sinnamariense, organic nitrogen utilisation, ectomycorrhiza.