African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12496

Full Length Research Paper

An investigation of meristem culture in Anchote [Coccinia abyssinica (Lam.) Cong.] under the influence of plant growth regulators and media strength

Solomon Abate Sisay
  • Solomon Abate Sisay
  • Department of Plant Science, College of Agriculture and Natural Resources, Madawalabu University, Bale Robe, Ethiopia.
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Firew Mekbib
  • Firew Mekbib
  • Department of Plant Sciences, College of Agriculture and Environmental Sciences, Haramaya University, Haramaya, Ethiopia.
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Endale Gebre
  • Endale Gebre
  • Ethiopian Institute of Agricultural Research, Addis Ababa, Ethiopia.
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  •  Received: 03 December 2018
  •  Accepted: 28 January 2019
  •  Published: 30 November 2020

Abstract

Considering the potential benefit of meristem culture in the mass production of quality propagation materials, an experiment was conducted to investigate meristem culture in KUWE (G1) and 223098 (G2) genotypes of anchote. Concentration levels of 6-benzylamimopurine (BAP) with and without gibberellic acid (GA3) and naphthalene acetic acid (NAA) for shoot induction while BAP and kinetin (Kn) for shoot multiplication were evaluated. Full and half nutrient-strength of Murashige and Skoog medium (FMS and HMS) with sole application of indole butyric acid (IBA) and indole acetic acid (IAA) were tested for root induction. About 80% (G2) and 71% (G1) of meristems induced shoots at 1 and 0.5 mg/L BAP, respectively. The highest shoot number (6.52), in G2, was recorded at 0.5 mg/L BAP while 0.5 mg/L BAP + 0.5 mg/L Kn produced 6.32 shoots in G1. FMS + 0.5 mg/L IBA was effective in 100% root induction in both genotypes. Survival rates of 58% (G1) and 42% (G2) were obtained. Generally, 0.5 mg/L BAP (G1) and 1 mg/L BAP (G) are best for shoot induction while 0.5 mg/L BAP (G2) and 0.5 mg/L BAP+0.5 mg/L Kn (G1) for multiple shoot development with best root formation on FMS+0.5 mg/L IBA.  

 

Key words: Direct regeneration, genotype, meristem explants, MS media, plant growth regulators (PGRs).