African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

The effect of exopolysaccharide biosynthesis and related enzyme activities of Grifola frondosa by the addition of ethanol extracts from traditional Chinese medicine, Gastrodia tuber

Xiao-bao Xu1, Tian-xiang Wu1* and Feng Wang2    
1School of Chemical Engineering, Guizhou University, Guizhou, Guiyang 550025, P. R. China. 2National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, P. R. China.    
Email: [email protected]

  •  Accepted: 03 February 2012
  •  Published: 21 February 2012

Abstract

Grifola frondosa is an edible medicinal polypore mushroom and its exopolysaccharide (EPS) has demonstrated immunomodulatory effects in many previous studies. After adding the Gastrodia tuber components in culture medium of G. frondosa, both EPS and biomass production were significantly increased. As a result, we detected two enzymes which are related to EPS synthesis. Our results suggested that the activity of α-phosphoglucomutase (α-PGM) increased, while the activity of phosphoglucose isomerase decreased. Mixture components of ethanol extract of Gastrodia tuber was more effective than pure gastrodine. A maximum dry cell weight of 138.5 mg l−1 and the EPS at 0.606 g l-1 were obtained when the unprocessed Gastrodia tuber culture was added.

 

Key words: Exopolysaccharide, phosphoglucomutase (PGM), phosphoglucose isomerase (PGI), Grifola frondosa, Gastrodia tuber.

Abbreviation

 

 

Abbreviations: EPS, Exopolysaccharide; α-PGM, α-phosphoglucomutase.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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