Full Length Research Paper
Abstract
The antioxidant activity of leaf extracts of Rosa canina from diverse localities of Tunisia were evaluated by ABTS and DPPH methods, whereas in those of essential oils and carotenoids extracts such activity was determined only by the ABTS method. Total phenols determined by the Folin method revealed that at Aindraham, samples showed a great variability of phenol content in contrast to those from Feija. After chemical analysis of the essential oils by gas chromatography (GC) and gas chromatography coupled to mass spectra (GC-MS), revealed that the oils of Feija were predominantly composed of palmitic acid, vitispirane, linoleic acid, lauric acid, myristic acid and phytol acetate, while in those samples from Aindraham predominated vitispirane, palmitic acid, linoleic acid and phytol acetate. Higher concentrations of b-carotene and lycopene were found in the samples from Aindraham after determination by high performance liquid chromatography (HPLC). All samples possess antioxidant activity, nevertheless much more significant in phenol extracts in contrast to the carotenoid extracts, which possess the lowest activity.
Key words: Secondary metabolites, biological activity, natural products.
Abbreviation
DPPH, 2,2'-Diphenyl-1-picrylhydrazyl radical; ABTS, 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid; GC, gas chromatography; GC-MS, gas chromatography coupled to mass spectra; HPLC, high performance liquid chromatography; MRSA, methicillin-resistant Staphylococcus aureus; IC50, sample concentration providing 50% inhibition; SWE, super-heated water extraction.
Copyright © 2024 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0
