African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Characterization of a new synthetic wheat – Aegilops biuncialis partial amphiploid

  Feiquan Tan1,2, Jianping Zhou2*, Zujun Yang2, Yong Zhang2, Li Pan2 and Zhenglong Ren1,2*    
  1State Key Laboratory for Plant Genetics and breeding, Sichuan Agricultural, University, Ya’an, Sichuan, P.R., China. 2Shcool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, Sichuan, P.R., China.  
Email: [email protected]; [email protected]

  •  Accepted: 17 April 2009
  •  Published: 20 July 2009

Abstract

 

The aim of the experiments was to identify the synthetic wheat – Aegilops biuncialisgermplasm Line 15-3-2 with 42 chromosomes. Morphologically, the spike of line 15-3-2 is intermediate to those of its wheat and Aegilops parents. Line 15-3-2 displays stable fertility and immunity to wheat powdery mildew and stripe rust. The seed storage protein electrophoresis showed that Line 15-3-2 presented almost all gliadin bands and a majority of glutenin subunits of its parents. Fluorescence in situhybridization (FISH) suggested that Line 15-3-2 contained all 14 D-genome chromosomes and chromosome 5U. Therefore, it could be concluded that Line 15-3-2 is a new synthetic wheat – A. biuncialis partial amphiploid, and could be used to transfer the disease resistance genes to wheat.

 

Key words: SDS-PAGE, A-PAGE, fluorescence in situ hybridization, wheat – Aegilops biuncialis partial amphiploid

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This article is published under the terms of the Creative Commons Attribution License 4.0

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