African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Protein landmarks for diversity assessment in wheat genotypes

M. F. Siddiqui* and N. Naz
Federal Seed Certification and Registration Department (FSC and RD), Islamabad, Pakistan.
Email: [email protected]

  •  Accepted: 06 March 2009
  •  Published: 31 May 2009

Abstract

 

Grain proteins from 10 Pakistani registered wheat genotypes were evaluated for diversity assessment based on sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS-PAGE). Genetic diversity was evaluated via UPGMA cluster analysis by constructing dendrogram of fractions of proteins, which were used for the calculation of similarity coefficients between these varieties. The greatest similarity (95%) was observed between Shahkar-95 and Mehran-89, while the lowest similarity (16%) was observed between Parwaz-94 and Abadgar-93.Adoption of this technology would be useful to plant protection regulatory systems, especially for plant variety identification and registration of new plant varieties, breeding programs and protection purposes.

 

Key words: SDS-PAGE, genetic diversity, coefficient of similarity.