African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Determination and expression of genes for resistance to blast (Magnaporthe oryza) in Basmati and non-Basmati indica rices (Oryza sativa L.)

  Naveen Kumar1 , D. Singh1*, S. Gupta3, A. Sirohi3, B. Ramesh4, Preeti Sirohi1, Parul Sirohi1, Atar Singh1, N. Kumar1, A. Kumar1, Rajendra Kumar2, R. Kumar3, J. Singh3, P. Kumar3, P. Chauhan3, Purushottam3 and S. Chand1  
   1Molecular Biology Laboratory, Department of Genetics and Plant Breeding, SVP University of Agriculture and Technology, Meerut, India-250 110. 2Department of Agri. Biotechnology, College of Agriculture, SVP University of Agriculture and Technology, Meerut, India-250 110. 3College of Biotechnology, SVP University of Agriculture and Technology, Meerut, India-250 110. 4Department of Genetics and Plant Breeding, CCS University, Meerut, India.
Email: [email protected]

  •  Accepted: 27 May 2013
  •  Published: 26 June 2013

Abstract

 

One hundred and twenty two (122) genotypes of Basmati and non-Basmati Indicarice genotypes were evaluated for expression of resistance against blast disease under induced epiphytotic conditions. Disease severity (%) and area under disease progress curve (AUDPC) parameters were used for screening the blast resistance. Only 13 genotypes expressed resistance against the blast disease. Nine genotypes carried blast resistance genes but, were susceptible under induced epiphytotic conditions. The rice genotype VLD-61 had no resistance genes; however, it expressed strong resistance against blast. An empirical breeding strategy for development of blast resistant improved varieties of rice was also discussed.

 

Key words: Magnaporthe oryzae, restriction digestion, molecular breeding, Basmati rice.

Abbreviation

Abbreviations: SFR, Super fine resolution; PCR, polymerase chain reaction; CTAB,cetyl trimethyl ammonium bromide; AUDPC, area under disease progress curve.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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