African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12488

Full Length Research Paper

Changes in the triacylglycerol content of flaxseeds during development using liquid chromatography- atmospheric pressure photoionization-mass spectrometry (LC-APPI-MS)

  Wahid Herchi1*, Hajer Trabelsi1, Hamadi Ben Salah2, Yuan Yuan Zhao3, Sadok Boukhchina1, Habib Kallel1 and Jonathan M. Curtis3
  1Laboratoire de Biochimie des lipides, Département de Biologie, Faculté des sciences de Tunis, 2092 ELmanar-Tunisie. 2Laboratoire des Cultures Industrielles, INRAT- Tunisia. 3Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
Email: [email protected]

  •  Accepted: 05 August 2011
  •  Published: 12 January 2012

Abstract

 

Changes in the composition of triacylglycerols (TAGs) of three varieties of flaxseeds (H52, O116 and P129) during development were investigated using non-aqueous reversed phase liquid chromatography coupled to atmospheric pressure photoionization-mass spectrometry (LC-APPI-MS). 17 species of triacylglycerols were found and their fatty acyl composition was confirmed by fragment ions in the mass spectra. The major forms observed were LnLnLn, LnLLn, LnOLn, LLLn, LnLnP, OOLn and OLLn. The highest change in these compounds occurred in the early development period for most TAGs. The most active period of triacylglycerol synthesis was between 7 and 35 days after flowering (DAF). LnLnLn exhibited the most significant increase during flaxseed development. At maturity, similar TAG profiles were found for the three samples.

 

Key words: Flaxseeds, seed development, triacylglycerols (TAGs), APPI, mass spectrometry.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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