Abstract

The genetic diversity of Pomacea canaliculata, collected from Los Banos (LB) in Philippines and Yuyao (YY), Taizhou (TZ), Fuzhou (FZ), Guangzhou (GZ), Nanning (NN), Kunming (KM) in China, was studied by using the inter simple sequence repeat (ISSR) technique. A total of 498 loci from 140 individuals were amplified with four selected ISSR primers and the percentage of polymorphic loci was 87.35%. At the species level, the Nei's gene diversity (H) was 0.3805 and the Shannon information diversity index (I) was 0.5607. A relatively high level of genetic differentiation among populations was detected based on Nei’s gene diversity analysis (G_st = 0.2001) and analysis of molecular variance (AMOVA) analysis (Φ_st= 0.0824), indicating the vast majority of genetic variation that occur within the populations. The limited genetic distance (0.0793) and correlation between genetic distance and geographic distance matrices (r = 0.5638, P > 0.5) indicated that, there was no significant geographic heterogeneity among these populations.

Key words: Pomacea canaliculata, inter simple sequence repeat (ISSR), genetic diversity, geographical populations.

Abbreviation

AMOVA, Analysis of molecular variance; ISSR, inter-simple sequence repeats; PCR, polymerase chain reaction; PPBs, percentage of polymorphic bands; RAPD, randomly amplified polymorphic DNA; RFLP, restriction fragment length polymorphism.