African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Isolation and characterization of Gram negative obligate and facultative alkalophilic Bacillus sp. from desert soil of Saudi Arabia

Abdulrahman S. A. Assaeedi1* and Gamal E. H. Osman1,2
  1Department of Biology, Faculty of Applied Sciences, Umm Al-Qura University, Makkah, Saudi Arabia. 2Agricultural Genetic Engineering Research Institute, Giza, Egypt.
Email: [email protected]

  •  Accepted: 25 April 2012
  •  Published: 22 May 2012

Abstract

 

Five new alkalophilic strains were isolated from soil samples collected from the western region of Saudi Arabia. They were identified by partial sequencing of 16s rRNA polymerase chain reaction (PCR) amplicons and morphological and biochemical characterization. Two of the isolates (number 2 and 3) were found to be facultative alkalophiles, while the other three (numbers 1, 4 and 5) were found to be obligate alkalophiles. Total protein profile analysis using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) revealed no significant differencesbetween isolates No. 2 and No. 3. On the other hand, there were major differences recognized in the banding pattern of the protein for isolate numbers 1, 4 and 5. All the isolates were 98% identical to Bacillus cohnii at the 16s rRNA gene.

 

Key words: Bacillus cohnii, 16SrRNA, Alkalophilic bacteria

Abbreviation

 

 

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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