African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Influence of 24- epibrassinolide on in vitro shootlets regeneration via direct organogenesis of Phaseolus vulgaris L.

Taha H. S.*
  • Taha H. S.*
  • Department of Plant Biotechnology, National Research Centre, Dokki, Cairo, Egypt.
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Nafie E. M.
  • Nafie E. M.
  • Department of Botany, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt.
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EL-Bahr M. K.
  • EL-Bahr M. K.
  • Department of Plant Biotechnology, National Research Centre, Dokki, Cairo, Egypt.
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Mansur R. M.
  • Mansur R. M.
  • Department of Botany, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo, Egypt.
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  •  Received: 30 October 2013
  •  Accepted: 25 February 2014
  •  Published: 09 July 2014

Abstract

This study aimed to optimize an in vitro shootlets regeneration system via direct organogenesis for Phaseolus vulgaris L. cv. Brunca using 24- epibrassinolide (24-Epi). The best medium for shootlets proliferation as well as growth parameters was MS supplemented with 2 mg L-1 TDZ + 0.1 mg L-1 24-Epi. Stem explants recorded the best results of shootlets proliferation percentage compared with leaf or root explants, respectively. MS medium supplemented with 2 mg L-1 TDZ + 0.1 mg L-1 24-Epi gave the best results of recovered shootlets (%); photosynthetic pigments, number of leaves, number of shootlets and shootlet length (cm). From the obtained results it can be recognized that, the behavior and trends of 24-Epi on in vitro P. vulgaris cultures media may be oriented and arranged to the behavior of auxins in the nutrient medium for roots formation and as a cytokinin in shootlets proliferation.

 

Key words: Phaseolus vulgaris L., 24- epibrassinolide, shootlets regeneration, direct organogenesis.

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