Construction of a full-length cDNA library and analysis of expressed sequence tags in white jute (Corchorus capsularis L.)

Aifen Tao; Xiaozhen Li; Jianmin Qi*; Pingping Fang; Lihui Lin; Jiantang Xu; Liwu Zhang; Jianmei Wu; Peiqing Lin

doi:10.5897/AJB2015.14619

African Journal of
Biotechnology

Abbreviation: Afr. J. Biotechnol.
Language: English
ISSN: 1684-5315
DOI: 10.5897/AJB
Start Year: 2002
Published Articles: 12487

Full Length Research Paper

Construction of a full-length cDNA library and analysis of expressed sequence tags in white jute (Corchorus capsularis L.)

Aifen Tao

Aifen Tao
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Provincial Key Laboratory of Crops by Design(Fujian Agriculture and Forestry University), Fuzhou 350002, P.R. China.
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Xiaozhen Li

Xiaozhen Li
Datong Township Government of Changting County, Longyan 366300, China.
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Jianmin Qi*

Jianmin Qi*
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Provincial Key Laboratory of Crops by Design(Fujian Agriculture and Forestry University), Fuzhou 350002, P.R. China.
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Pingping Fang

Pingping Fang
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Provincial Key Laboratory of Crops by Design(Fujian Agriculture and Forestry University), Fuzhou 350002, P.R. China.
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Lihui Lin

Lihui Lin
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Provincial Key Laboratory of Crops by Design(Fujian Agriculture and Forestry University), Fuzhou 350002, P.R. China.
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Jiantang Xu

Jiantang Xu
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Provincial Key Laboratory of Crops by Design(Fujian Agriculture and Forestry University), Fuzhou 350002, P.R. China.
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Liwu Zhang

Liwu Zhang
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Provincial Key Laboratory of Crops by Design(Fujian Agriculture and Forestry University), Fuzhou 350002, P.R. China.
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Jianmei Wu

Jianmei Wu
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Provincial Key Laboratory of Crops by Design(Fujian Agriculture and Forestry University), Fuzhou 350002, P.R. China.
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Peiqing Lin

Peiqing Lin
Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Provincial Key Laboratory of Crops by Design(Fujian Agriculture and Forestry University), Fuzhou 350002, P.R. China.
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Article Number - CF272B553541
Vol.14(23), pp. 1928-1935 , June 2015
https://doi.org/10.5897/AJB2015.14619

Received: 03 April 2015
Accepted: 02 June 2015
Published: 10 June 2015

Abstract

White jute (Corchorus capsularis L.) is recognized as an important industrial raw material fibre crop owing to its elite characters. However, little information is known about its molecular basis and genomics. In this study, a complementary DNA library of white jute was constructed and expressed sequence tags (ESTs) were characterized. The titers of original and amplified libraries were 2.32 × 10⁷ and 1.07 × 10⁹ pfu/mL, respectively. The recombinant frequency was 98.3% in the library. Most of the sequences ranged from 500 to 1500 bp with an average length of 750 bp. Results show 203 (73%) ESTs exhibited significant similarity with known or putative functional nucleotide sequences in the GenBank databases. Cluster analysis allowed the identification of 61 unique sequences. These genes were classified into six types by Gene Ontology (GO) annotation. The results also indicated that unigenes of C. capsularis have higher homology to Populus trichocarpa, Ricinus communis and Corchorus olitorius. This report will provide a valuable resource for the further investigations in the gene cloning, transcription or expression for white jute.

Key words: White jute, Corchorus capsularis, cDNA library, construction, ESTs, analysis.

Abbreviation

ESTs, Expressed sequence tags; GO, gene ontology; cDNA, complementary DNA; SMART, switching mechanism at 5' end of the RNA transcript; mRNA, messenger RNA; SSR, simple sequence repeat; BGI, Beijing genomics institution; NCBI, national center for biotechnology; PVPP, polyvinylpyrrolidone.