African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12368

Full Length Research Paper

Adenine sulfate and glutamine enhanced shoots multiplication in Jatropha curcas L. a potent biofuel plant

Ismail A. Mohammed
  • Ismail A. Mohammed
  • Department of Botany and Agricultural Biotechnology, Faculty of Agriculture, University of Khartoum, Khartoum, Sudan.
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Suguru Tsuchimoto
  • Suguru Tsuchimoto
  • Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan.
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Hiroe Sakai
  • Hiroe Sakai
  • Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan.
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Naoki Wada
  • Naoki Wada
  • Graduate School of Technology, Industrial and Social Sciences, Tokushima University, Tokushima 770-0814, Japan.
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Kiichi Fukui
  • Kiichi Fukui
  • Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan.
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  •  Received: 27 May 2021
  •  Accepted: 31 August 2021
  •  Published: 30 September 2021

Abstract

Jatropha is a drought-tolerant plant producing seed oil for the biodiesel. Limitation to the development of jatropha is unavailability of high-yielding varieties and efficient in vitro regeneration system which is required for micropropagation. In this study, in vitro regeneration system from jatropha juvenile cotyledon was established. Firstly, concentrations of hormones in the MS medium were optimized and it was found that 1.5 mgL-1 benzyl adenine (BA) + 0.05 mgL-1 indole-3-butyric acid (IBA) + 0.5 mgL-1 thidiazuron (TDZ) turned out to be the best for shoot induction (3.82 ± 0.18 shoots/explant). Secondly, shoot induction medium was fortified with different concentrations of glutamine and adenine sulfate. It was found that 25 mgL-1 each of glutamine and adenine sulfate was the most effective, resulting to 9.09 ± 0.37 shoots/explant and 93.0% regeneration frequency. Regenerated shoots were cultured on medium containing 0.5 mgL-1 BA and different concentrations of gibberellic acid (GA3), 0.5 mgL-1 GA3 with 0.5 mgL-1 BA  were found to be the best for shoot elongation (2.13 ± 0.18 cm). The highest frequency of root (40%) was observed on the medium with 0.5 mgL-1 IBA. The established procedures will be useful for the mass propagation and genetic transformation of elite jatropha genotypes.
 
Key words: Adenine sulfate (Ads), benzyl adenine (BA), indole-3-butyric acid (IBA), in vitro regeneration, glutamine (Gln).