African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12193

Full Length Research Paper

Micronuclei in red blood cells of armored catfish Hypostomus plecotomus exposed to potassium dichromate

Carlos Augusto Borba Meyer Normann1, José Cláudio Fonseca Moreira2 and Valesca Veiga Cardoso1*
  1Centro Universitário Metodista - Porto Alegre - RS –Brazil. 2Department of Biochemistry-ICBS; UFRGS - Porto Alegre - RS - Brazil.
Email: [email protected]

  •  Accepted: 22 February 2008
  •  Published: 30 April 2008

Abstract

 

The present work aims to evaluate the impact of potassium dichromate in armored catfishes’ (Hypostomus plecotomus) erythropoiesis, using piscine micronucleus testArmored catfishes (n = 30) were subjected to 12 mg/L of potassium dichromate, with an equal control group (n = 30). For each 2,000 red blood cells of animals subjected to treatment, it was possible to observe 8.25 ± 0.02% cells with micronuclei and 17 ± 0.005 mg/kg of total chrome in tissues, detected through atomic absorption spectrophotometry. In the control group, the average was 0.75 ± 0.03% for micronuclei and 1.08 ± 0.04 mg/kg of chrome in tissues. The results confirm the clastogenic effect of potassium dichromate. The sensibility to this ion and the economic relevance of H. plecotomus show that armored catfish can be used to monitor acute effects of metallic pollutant spills in freshwater.

 

Key words: Piscine micronucleus test, aquatic pollution, potassium dichromate,Hypostomus plecotomus.