African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Genetic variation of calsarcin-1 gene and association with carcass traits in 3 Chinese indigenous cattle

Dapeng Yang1, Linsen Zan1,2*, Hongbao Wang1,2, Yun Ma1, Wanqiang Tian1and Yingying Zhang1
  1College of Animal Science and Technology, Northwest A and F University, Yangling, Shaanxi 712100, P. R. China. 2National Beef Cattle Improvement Center, Yangling, Shaanxi 712100, P. R. China.
Email: [email protected]

  •  Accepted: 27 May 2009
  •  Published: 17 June 2009

Abstract

 

This study aimed to investigate polymorphisms of Calsarcin-1 gene and evaluate its effect on carcass traits in 429 samples of 3 Chinese indigenous cattle breeds, namely, Luxi (LX), Nanyang (NY) and Jiaxianred (JXR) breeds, PCR products with a 320 bp fragment of the Calsarcin-1 gene spanning over a part of intron 2, complete exon 3 and a part of intron 4 were amplified and sequenced. A synonymous alteration (NW_001495138:g.16718C>A) in the exon 3 region of the calsarcin-1 gene was detected and PCR-SSCP method was then developed to genotype all of the individuals. The results showed that the allele frequencies of A/C in LX, NY and IXR breeds were 0.1116/0.8884, 0.1591/0.8409 and 0.0947/0.9053, respectively. Least squares analysis revealed a significant effect (P<0.05) of genotypes on backfat thickness and tenderness of the animals, with the AC and AA genotype being favourable compared to the CC genotype.

 

Key words: Calsarcin-1 gene, carcass traits, cattle, SNP.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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