African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Molecular marker analysis to differentiate a clonal selection of Centennial Seedless grapevine

  Manisha P. Shinde, Anuradha Upadhyay*, Lalitkumar B. Aher and G. S. Karibasappa      
National Research Centre for Grapes, Solapur Road, Pune -412307, Maharashtra, India.
Email: [email protected]

  •  Accepted: 01 March 2013
  •  Published: 03 April 2013

Abstract

 

Microsatellite and amplified fragment length polymorphism (AFLP) markers were used to differentiate Manjari Naveen, a clonal selection of Centennial Seedless variety of grape. Twenty one (21) microsatellite primers could not detect variation between parent variety and its clone. AFLP analysis with 40 primer combinations generated 1093 markers. Three AFLP markers were polymorphic; two markers were present only in Centennial Seedless, whereas one was present only in Manjari Naveen. These markers will be useful for establishing genetic identity, variety registration and protection of breeder’s right.

 

Key words: Vitis vinifera, AFLP, microsatellites, clonal selection.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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