African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Genetics similarity among four breeds of goat in Saudi Arabia detected by random amplified polymorphic DNA marker

Jamal S. M. Sabir1, Mohammed H. Z. Mutawakil1,  Amr A. El-Hanafy1,2 and Mohamed M. Ahmed1,2*    
1Department of Biological Sciences, Faculty of Science, P. O. Box 80203, King Abdulaziz University, Jeddah, 21589, Saudi Arabia. 2Department of Nucleic Acid Research, Genetic Engineering and Biotechnology Research Institute, City for Scientific Researches andTechnology Application, Borg EL-Arab, P. O .box. 21934, Alexandria, Egypt.    
Email: [email protected]

  •  Accepted: 14 February 2012
  •  Published: 28 February 2012

Abstract

Phylogeny analysis using random amplified polymorphic DNA (RAPD) markers was performed for studying genetic variation in four Saudi Arabia goat breeds, namely: Harri, Ardi, Habsi and Masri. Six goats from Harri breed, four each from both Ardi and Habsi breeds and five from Masri breed were used for the experiment. Four different 10 bp RAPD primers were employed for the polymerase chain reaction (PCR). The results obtained showed that Harri and Ardi goat breeds lay in the same group and share about 73.5% genetic similarity, while Habsi and Masri goat breeds were closer to each other more than the previous two breeds, where they share about 82.5% of genetic similarity.

 

Key words: Goats, breeds, RAPD, genetic similarity.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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