Abstract

Thin cell layer (TCL) technology originated almost 30 years ago with the controlled development of flowers, roots, shoots and somatic embryos on tobacco pedicel longitudinal TCLs. Since then TCLs have been successfully used in the micropropagation of many ornamental plant species whose previous in vitroregeneration was not successful using conventional methods. This review examines the fundamentals behind TCLs, and their application in ornamental plant micropropagation and transformation.

Key words: Chrysanthemum, lily, somatic embryogenesis, thin cell layer, tobacco, transformation. 

Corrigendum

Some of the figures  (in Figure 1) were published in papers that were published at the same time that the review was published, which was impossible to report at that time. The figures were previously published in:

Teixeira da Silva, J.A., Fukai, S. (2003) Chrysanthemum organogenesis through thin cell layer technology and plant growth regulator control. Asian Journal of Plant Sciences 2(6): 505-514.

Jaime A. Teixeira da Silva (2003) Thin cell layer technology for induced response and control of rhizogenesis in chrysanthemum. Plant Growth Regulation January 2003, Volume 39, Issue 1, pp 67-76.