African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Molecular cloning and characterization of the plasma membrane aquaporin gene (OvPIP) from Orychophragmus violaceus

ShiZhan Du1, Qi-lin Dai1, ChenChen3 Bin Feng2, Ting-ting Liu2, Xia Tian2, Yuan-ya Gong2, Ying-kun Sun 2 and JinWang1,2*
1Plant Genomics Research Center, Mianyang Normal College, Mianyang, Sichuan, 621000, China. 2Key Laboratory for Nuclear Waste Treatment and Environmental Safety (SWUST), Commission of Science, Technology and Industry for National Defence, Southwest University of Science and Technology, Mianyang, Sichuan, 621000, China. 3School of Pharmacy, Fudan University, Shanghai 200030, China.
Email: [email protected].

  •  Accepted: 28 March 2011
  •  Published: 09 May 2011

Abstract

A plasma membrane aquaporin gene (OvPIP) from Orychophragmus violaceus(O. violaceus) was cloned. The full-length cDNA of O. violaceus gene (OvPIP) was 1314 bp and contained 1188 open reading frame encoding a protein of 395 amino acids. Homology analysis revealed that OvPIP strongly resembled other PIP genes. Southern blot analysis indicated that OvPIP gene was present in O. violaceusgenome, and quantitative real-time PCR (QRT-PCR) analysis revealed that OvPIPtranscript level was most abundant after 12 h treatment with 200 mM sodium chloride. Our studies suggested that OvPIP is a member of the family of the recently cloned plant plasma membrane aquaporins gene.

 

Key words: Orychophragmus violaceus, plasma membrane, tonoplast aquaporins gene, RACE-PCR, quantitative real-time PCR.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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