African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Extraction and analysis of tea (Camellia sinensis) seed oil from different clones in Kenya

Kelvin Omondi George1,2, Thomas Kinyanjui2*, John Wanyoko3, Okong’o Kelvin Moseti3 and Francis Wachira3
  1Bidco Oil Refineries Limited, P. O. Box 7029 Nakuru, Kenya. 2Chemistry Department, Egerton University, P. O. Box 536 Njoro, Nakuru, Kenya. 3Tea Research Foundation of Kenya, P. O. Box 820-20200, Kericho, Kenya.
Email: [email protected]

  •  Accepted: 22 November 2012
  •  Published: 20 February 2013

Abstract

 

Kenyan tea (Camellia sinensis) is widely grown for its leaves and is commercialized as black tea. Product diversification and value addition is currently an area of great interest. This study provides data on the physico-chemical properties of Kenyan tea seed oil from selected clones of tea seeds to ascertain its potential applications. Soxhlet extraction using hexane was employed to obtain tea seed oil followed by chemical analysis to assess its properties. Oil yield, iodine value, saponification value, peroxide value, free fatty acids, total polyphenols and antioxidant activity were determined. The oil yields ranged between 16 to 25% w/w. Iodine value was in the range of 86 to 91 g I2/100 g, peroxide value < 3.5 meq O2/kg, saponification value between 182 to 187 mg KOH/g, free fatty acid < 1.5% oleic acid, total polyphenols 0.036 to 0.043 mg/L gallic acid and antioxidant activity of between 14 to 21% 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. Tea seed oil is stable and can be a potential source of natural antioxidants.

 

Key words: Tea seed oil, iodine value, saponification value, peroxide value, free fatty acids, total polyphenols, antioxidant activity.