African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Nitrogen supplements effect on amylase production by Aspergillus niger using cassava whey medium

  C. E. Oshoma1*, E. E. Imarhiagbe2, M. J. Ikenebomeh1 and H. E. Eigbaredon1  
  1Department of Microbiology, University of Benin, P.M.B. 1154, Benin City, Nigeria. 2Green Consultants Ltd, Edo State Environmental Laboratory, House Annex, Sapele Road, Benin City, Nigeria.
Email: [email protected]

  •  Accepted: 27 April 2009
  •  Published: 01 February 2010

Abstract

 

The production of amylase by Aspergillus niger on three cassava whey media in liquid shake culture was compared. The supplemented cassava whey (SCW) medium exhibited gave amylase activity of 495 U/ml. Biomass cropped was 1.63 g/l in the SCW medium. Yeast extract employed as a nitrogen supplement increased biomass yield of A. niger to 2.75 g/l with maximum amylase activity of 643 U/ml. Sodium nitrate (NaNO3) as nitrogen supplement had the lowest biomass yield of 0.77 g/l and amylase activity of 206 U/ml. Thus yeast extract as nitrogen supplement of cassava whey medium supported maximum production of amylase and biomass of A. niger.

 

Key words: Amylase, cassava whey, Aspergillus niger, yeast extract.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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