Full Length Research Paper
Abstract
In this present study, we established an efficient Agrobacterium-transformationsystem using an inducible expression vector co-cultivated with lisianthus leaf explants. To explore the potential application of the inducible system pJCGLOX in transgenic lisianthus (Eustoma grandiflorum cv. LisaBlue). Arabidopsis FLOWERING LOCUS T (FT) gene was employed as the target gene. Genomic polymerase chain reaction (PCR) and southern blotting confirmed the presence of this transgene in the genomes of several kanamycin-resistant lines. Transcription of FT was shown to be activated in these transformants after inducing treatment, demonstrating efficacy of the inducible systems in this kind of flower.
Key words: Genetic transformation, inducible gene expression. Eustoma grandiflorum, CRE-lox recombination.
Abbreviation
6-BA, 6-Benzylaminopurin; AS, Acetosyringone; CTAB,hexadecyltrimethylammonium bromide; GFP, green fluorescent protein; HSP, heat shock protein; IBA, indole-3-butyric acid; MS medium, Murashige and Skoog medium; NAA, naphthalene-acetic acid; PCR, polymerase chain reaction.
*Both authors contributed equally to the work.
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