Full Length Research Paper
Abstract
This study was carried out to evaluate the effect of long-term natural aging ongermination ability and several biochemical characteristics regarding soluble sugars and polyphenol matter contents and radical scavenging activity of Periplocaangustifolia Labill. (Asclepiadaceae) stored seeds for 1, 3, 7, 10, 11 and 15-years, dry storage compared to freshly collected seeds. The long-term aging caused an important decrease of germination percentage, seed vigor index, seeds’ viability,moisture content and seed vigor except for seeds stored for seven-years. The latter showed the highest percentages of germination and viability, seed vigor index and seed vigor under a 7.3% moisture content when compared to the oldest seeds (15-years old) which presents the lowest moisture content. In our study, aged seeds showed the lowest radical scavenging percentage activity and amounts of polyphenol, keeping free radicals and peroxides at high levels causing thereby seeds deterioration.P. angustifolia seeds undergo a process of after-ripening under the storage conditions, possibly depending on the low, but steady water loss down to an optimal storage water content of approx. 7.3%, thereafter undergoing some deterioration as indicated by reduced amounts of soluble sugars by polyphenol contents and experimentally tested antioxidant activities, which is in line with increased membrane leakages as indicated by increased electrical conductivities of solution from experimentally soaked seeds.
Key words: Periploca angustifolia Labill, polyphenol, germination.
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