African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Effect of nitrogen and phosphate limitation on utilization of bitumen and production of bitu-oil and gas by a bacterial consortium

J. K. Oloke1*, E. A.  Adebayo1 and D. A. Aina2
  1Department of Pure and Applied Biology, P. M. B. 4000, LAUTECH, Ogbomoso, Nigeria. 2Departments of Biosciences and Biotechnology, Babcock, University, Ilisan, Ogun State, Nigeria.
Email: [email protected]

  •  Accepted: 03 October 2009
  •  Published: 15 December 2009

Abstract

 

Five strains of bacteria; Pseudomonas fragi, Streptococcus zymogenes,Pseudomonas aeruginosa, Pseudomonas fluorescens and Bacillus macerans, capable of utilizing bitumen as source of carbon and energy were isolated from water sample obtained from bitumen producing area, Agbabu, Ondo state, Nigeria. The degradation of bitumen was found to be associated with the production of carbon (IV) oxide, natural gas and oil. As a result of using nitrogen limited and phosphate limited media, 1750 and 1250 cm3 of gas and 0.95 and 0.85 g/l of oil were obtained respectively. Nitrogen and phosphate limitation have profound effect on bitu-oil and gas production.

 

Key words: Bitumen, biodegradation, phosphate limited, nitrogen, bitu-oil.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

Back to Vol. 8 No. 24
Back to articles
  • Articles on Google by:
SUBSCRIBE TO RSS
SUBMIT MANUSCRIPT
CONFERENCES