African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Complete nucleotide sequencing, SNP identification and characterization of SRY gene in Indian Sangamneri goat

Prashant, D. S. Gour, P. P. Dubey, A. Jain, D. K. Nanda, B. K. Joshi and Dinesh Kumar*
Genes and Genetic Resources Molecular Analysis Lab, National Bureau of Animal Genetic Resources, Karnal –132001, India
Email: [email protected]

  •  Accepted: 27 March 2009
  •  Published: 06 July 2009

Abstract

 

The SRY gene is responsible for testis determination in mammals. It codes for a protein with a DNA binding domain similar to those found in high-mobility group protein (HMG box). Mutation in this gene leads to Gonadal Dysgenesis an abnormal sexual phenotype described in human, cattle, horse, and river buffalo. Here we report PCR-SSCP analysis and sequencing of coding region of SRY gene in Sangamneri goat (Capra hircus) using 40 male goats (bucks) samples. Polymorphism was detected in only one sample indicating that a major portion of SRY gene is highly conserved in this breed.

 

Key words: Goat, HMG box, SSCP, SRY gene.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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